We've done dual labelling on Ventana immunostainers.  One that comes to
mind is Ki67/CD31.  We did HIER in 1mM EDTA for 3" in Biocare's decloaking
chamber followed Ki67 (1:75 of Zymed's/28")using DAB detection with AB
block and amplification but no counterstain.  We followed that with CD31
staining (1 drop of Neomarker's/4")using Alkaline Phosphotase Red with
amplification and counterstain.

This is just an example; we've done other combo's.  Let us know if you have
particular questions and maybe we can help you out.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Brennan, Liam [mailto:Liam.Brennan@bll.n-i.nhs.uk]
Sent: Thursday, July 17, 2003 7:37 AM
To: 'HISTONET'
Subject: Double Immuno-staining


17/7/03

Hi- Anyone out there doing double or triple immuno staining on the Ventana
NexES? What detection system's are you using? Willing to share your
protocols?

Thanks	Liam Brennan
		Belfast City Hospital
		Northern Ireland