RE: Double Immuno-staining
|From:||"Sebree Linda A." (by way of Histonet)|
We've done dual labelling on Ventana immunostainers. One that comes to
mind is Ki67/CD31. We did HIER in 1mM EDTA for 3" in Biocare's decloaking
chamber followed Ki67 (1:75 of Zymed's/28")using DAB detection with AB
block and amplification but no counterstain. We followed that with CD31
staining (1 drop of Neomarker's/4")using Alkaline Phosphotase Red with
amplification and counterstain.
This is just an example; we've done other combo's. Let us know if you have
particular questions and maybe we can help you out.
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: Brennan, Liam [mailto:Liam.Brennan@bll.n-i.nhs.uk]
Sent: Thursday, July 17, 2003 7:37 AM
Subject: Double Immuno-staining
Hi- Anyone out there doing double or triple immuno staining on the Ventana
NexES? What detection system's are you using? Willing to share your
Thanks Liam Brennan
Belfast City Hospital
<< Previous Message | Next Message >>