RE: Cross Contamination
We wiped the forceps between each tissue block. A big source of cross
contamination is waterbath. We took a Kimwipe and skimmed the surface
between cases and even each blocks at times, plus kept our fingers
(exfoliating cells) OUT of the waterbath water.
At 06:39 PM 7/9/2003 +0000, you wrote:
>Don't rule out cross-contamination at the grossing board. Do you ever have
>problems with tissues that never touch the grossing board ? I also prefer to
>wipe my forceps with a Kimwipe before and after each embedding to prevent
>forcep metatasis. Hope this helps. Tom Truscott USDA-ARS Pullman, WA
>>From: "LaFriniere, Mike"
>>To: "'DMBCMP@aol.com'" , email@example.com
>>Subject: RE: Cross Contamination
>>Date: Mon, 07 Jul 2003 13:20:37 -0400
>>We use a "Bacti-Cinerator" works well and replaces the open flame
>>Michael LaFriniere PA, HT(ASCP)
>>Chattanooga TN 37404
>>From: DMBCMP@aol.com [mailto:DMBCMP@aol.com]
>>Sent: Monday, July 07, 2003 12:43 PM
>>Subject: Cross Contamination
>>My lab has an ongoing problem of cross-contamination, which I believe
>>during embedding. Our hospital does not allow the use of an open flame in
>>the Histology lab. Flaming the forceps with a bunsen burner would no doubt
>>solve the problem but since that is not an option, does anyone have a
>>We have tried non-grooved forceps, but were unable to grasp the tiny pieces
>>of tissue. The "forceps warmer" on the embedding centre is only barely hot
>>enough to keep the forceps warm for a few moments. We switch forceps back
>>forth during embedding.
>>I would appreciate any suggestions. Thanks.
>>Dannie Blake (HT)
>>Histo Technical Specialist
>>Fresno Community Hospital
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Veterinary Molecular Biology - Marsh Lab
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