HIER and type II collagen


Hello all!

We have been using antibody against type II collagen in mouse tissue fixed in Dent's and paraffin embedded successfully but have started using HIER with pressure cooker and citrate buffer and this antibody doesn't seem to be working (no staining in areas of limb cartilage that have always stained before).  All other antibodies seem to love the AR (sections on same slide, incubated with antibodies against neurofilament, against myosin, against versican, all lit up beautifully).  
Does anybody know of a particular problem with 11-116B3 or other antibodies against type II collagen?
As always, TIA
Maureen Loomer
M.S. candidate (Cell Biology)
East Carolina University

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