...."boil under Reflux"....???????

From:Bruce Abaloz

Hello, back again with another question....first I want to Thank everyone who responded to my query RE: Sirius Red/Direct Red 80. 
NOW, we are wanting to do the Nile Blue Sulphate method for Acidic & Neutral Lipids...preparation of Stain is to ....."boil under Relux for four hours"....am in the 'dark' as to what this means...can someone PLEASE advise?? 
Thanks again,

Bruce in OZ.
BRUCE ABALOZ                           PH:61383446282
HISTOLOGIST                            FAX:61383447909 
DEPT.of ZOOLOGY                        EMAIL: brucea@unimelb.edu.au
VICTORIA.AUSTRALIA 3010           

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