zinc fixative and sma


Hello Histonetters

it has been discussed many times about zinc fixative (=formalin-free) ->
histonet archive .We like to use it to stain surface markers, e.g. CD3,
CD4, CD8, CD20 in monkey tissue, since in formalin fixed paraffin embedded
tissue it is much more difficult to get an acceptable staining intensity
without background.
But now we would like to stain for smooth muscle actin in zinc fixed
material. So far the results are disappointing because of high background.
In FFPE the staining looks nice and clean. Any explanation for that ?
Thank you for your help.

Antje Marcantonio
Novartis Pharma AG, Basel CH

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