xylene problems
Dear Histonetters,
I would like to ask you a question about clearing
the slides. I am staining them with Masson trichrome and when I try to clear
colored slides wtih xylene (aftre alcohole) the colors are becoming very dark
(even black) .
Can I do not clear them with xylene, but mount
after dehydrating in alcohol?
Please help, I am first time in staining.
Thanks in advance ,
Rafael
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