staining protocol for CD31 and vWF.

From:Raj Rao

Hello All,
  I am interested in a working staining protocol for CD31(PECAM1) and 
vWF (von willebrand factor). I am in the process of standardazing the 
protocol using HUVEC cells adhered in chamber slides. Any pointers in 
staining conditions is appreciated. I am using primary antibodies CD31 
(Sigma) and vWF (DAKO). Secondaries are the Alexa set of dyes from 
Molecular Probes.


Raj R. Rao, Ph.D.,
Postdoctoral Research Fellow
Georgia Biobusiness Center (Rm 172)
111, Riverbend Road    Ph: (706)613-9878 (172/175)
University of Georgia  Fx: (706)613-9879
Athens, GA 30602

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