my last question appeared to have been too specific (the diI /
immunocytochemistry question), so here is one that is more general.  we
have tissue that has sat in paraformaldehyde for 6 weeks (while the diI
tracer transported).  we now want to do immunocytochemical processing to

visualize a peptide we are interested in.  would antigen unmasking be
likely to help, and if so, can anybody reccomend an easy beginning
unmasking method?

thanks,
rick