Rafael,
Does this problem occur while the sections are covered with xylene and when mounted? Sometimes when the xylene evaporates and the section is dry the impression is that the colours are different, this disappears when the sections are wetted again. Give some more details please.
Best regards
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, friut flies like a banana"
>From: Rafael Badalyan
>To: histonet@pathology.swmed.edu
>Subject: xylene problems
>Date: Wed, 17 Jul 2002 00:15:47 +0500
>
>Dear Histonetters,
>I would like to ask you a question about clearing the slides. I am staining them with Masson trichrome and when I try to clear colored slides wtih xylene (aftre alcohole) the colors are becoming very dark (even black) .
>Can I do not clear them with xylene, but mount after dehydrating in alcohol?
>
>Please help, I am first time in staining.
>Thanks in advance ,
>Rafael