We use 30% sucrose.
Steve


                                                                                                                                              
                    "CRME (Criss                                                                                                              
                    Meligro)"            To:     "'histonet@pathology.swmed.edu'"                               
                                    Subject:     frozen sections fixed in formalin                                                       
                                                                                                                                              
                    25/07/2002                                                                                                                
                    19:56                                                                                                                     
                                                                                                                                              
                                                                                                                                              




We have just received some tissue for Oil Red O that has been fixed in
formalin... I know that the water must be exchanged with a sucrose solution
but don't remember the concentrations that work best.... currently we have
it in 10%.  Any suggestions appreciated.