Re: Stain for Alzheimer's disease

From:Anila Syed

Our lovely lab manager, who left recently, published this a while ago, it might be helpful for you:
Litchfield S, Nagy Z. Related Articles
New temperature modification makes the Bielschowsky silver stain reproducible.
Acta Neuropathol (Berl). 2001 Jan;101(1):17-21.
PMID: 11194935 [PubMed - indexed for MEDLINE]
Best wishes

- Original Message -----
Sent: Tuesday, July 30, 2002 9:49 AM
Subject: RE: Stain for Alzheimer's disease

Apologies for anonymous posting.
Andy Shand
-----Original Message-----
From: []
Sent: 30 July 2002 09:21
Subject: RE: Stain for Alzheimer's disease

Remember that all neuropath silvers are, to say the least, capricious (bitter experience).  They can be used to demonstrate anything by varying time, temp, pH etc.  Use the method as a guide but be prepared to vary to suit local conditions.  If possible get a method from a lab with the same water hardness.
Another avenue worth exploring is immuno.  It's easier and produces good results.  Antibodies worth looking at include PHF-Tau-AT8, Alzheimer Precursor Protein A4, Beta Amyloid and Beta Amyloid Peptide.
-----Original Message-----
From: Histology, Metro Lab []
Sent: 29 July 2002 20:21
To: histonet posting address
Subject: Stain for Alzheimer's disease

I am looking for a stain to show the neurofibrillary changes found in Alzheimer's disease.  A couple of findings from our pathologist was the Cross Modified Palmgren stain, with no reference on how to prepare the stain and the Bielschowski's technique.  Anyone have any suggestions?
Deb Croegaert
Metro Labs
Davenport< Iowa

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