Re: Stain for Alzheimer's disease
Our lovely lab manager, who left recently,
published this a while ago, it might be helpful for you:
New temperature modification makes the Bielschowsky silver stain
Acta Neuropathol (Berl). 2001
PMID: 11194935 [PubMed - indexed for MEDLINE]
- Original Message -----
Sent: Tuesday, July 30, 2002 9:49
Subject: RE: Stain for Alzheimer's
Apologies for anonymous
Remember that all neuropath silvers are,
to say the least, capricious
(bitter experience). They can be used to demonstrate anything by
varying time, temp, pH etc. Use the method as a guide but be
prepared to vary to suit local conditions. If possible get a
method from a lab with the same water
Another avenue worth exploring is immuno.
It's easier and produces good results. Antibodies worth looking
at include PHF-Tau-AT8, Alzheimer Precursor Protein A4, Beta Amyloid
and Beta Amyloid Peptide.
I am looking for a stain to show
the neurofibrillary changes found in Alzheimer's
disease. A couple of findings from our pathologist was
the Cross Modified Palmgren stain, with no reference on how to
prepare the stain and the Bielschowski's technique. Anyone
have any suggestions?
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