Vinegar is used here,,,,
----- Original Message -----
From: "Jennings-Siena, Debbie" 
To: "Shaun Kay" ; 
Sent: Thursday, July 18, 2002 11:08 AM
Subject: RE: Setting ink on tissues


>
> Use a low pH, such as an weak acid.  That is why decal solution works
well.
> Debbie J. Siena, HT(ASCP)QIHC
> Histopathology Manager
> Baylor University Medical Center
> 3500 Gaston Ave.
> Dallas, TX 75246
> 214-820-2465 vm
> 214-820-4110 fax
> debbiesi@baylordallas.edu
>
>
> -----Original Message-----
> From: Shaun Kay [mailto:skay@dcdel.com.au]
> Sent: Wednesday, July 17, 2002 6:59 PM
> To: histonet@pathology.swmed.edu
> Subject: Re: Setting ink on tissues
>
>
> Could you tell us what pH we are talking about as I have recently had a
> problem with the india ink staying on the tissue during processing.  Its
> been fine for 15 years and now starts to play up
> Mr Shaun Kay
> Chief Laboratory Scientist
> Davies Campbell deLambert Pathology
> 2 Leeds Street
> Rhodes
> NSW 2138
> Australia
> Tel 02 9736 2555
> or skay@dcdel.com.au
> ----- Original Message -----
> From: "Jennings-Siena, Debbie" 
> To: "Charles.Embrey" ; "Linda McDonald"
> 
> Cc: 
> Sent: Thursday, July 18, 2002 7:09 AM
> Subject: RE: Setting ink on tissues
>
>
> >
> > It is my understanding that it is the pH that sets the India Ink.  I
have
> been using out of date decal solution and it works great.
> > Debbie J. Siena, HT(ASCP)QIHC
> > Histopathology Manager
> > Baylor University Medical Center
> > 3500 Gaston Ave.
> > Dallas, TX 75246
> > 214-820-2465 vm
> > 214-820-4110 fax
> > debbiesi@baylordallas.edu
> >
> >
> > -----Original Message-----
> > From: Charles.Embrey [mailto:Charles.Embrey@carle.com]
> > Sent: Wednesday, July 17, 2002 11:06 AM
> > To: 'Linda McDonald'
> > Cc: 'histonet@pathology.swmed.edu'
> > Subject: RE: Setting ink on tissues
> >
> >
> > Vinegar (dilute GAA) has been use for decades to set dyes in the
clothing
> > industry.  It is the GAA in the bouin's solution as well as the picric
> acid
> > that sets the ink so just making dilute GAA is much safer and cost
> > effective.  I keep a spray bottle of 5% GAA next to my cutting board to
> > spray the specimens.  No mess little waste.
> > Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
> > Histology Manager
> > Carle Clinic
> > Urbana, IL
> >
> >
> > -----Original Message-----
> > From: Linda McDonald [mailto:mcdonal1@ccf.org]
> > Sent: Wednesday, July 17, 2002 10:44 AM
> > To: histonet@pathology.swmed.edu
> > Subject: Setting ink on tissues
> >
> >
> > Hello!  Question for the netters:  Who or when or how did it come about
to
> > use a dilute glacial acetic acid solution to help set ink on tissues.
> This
> > is used in place of dunking in Bouin's.  Anybody know?
> > Thanks,
> > Linda McDonald
> > Cleveland Clinic Health Systems
> > Cleveland, OH
> >
> >
> >
> >
> >
> >
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>
> This e-mail, facsimile, or letter and any files or attachments
> transmitted with it contains information that is confidential and
> privileged. This information is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If you are
> the intended recipient, further disclosures are prohibited without
> proper authorization. If you are not the intended recipient, any
> disclosure, copying, printing, or use of this information is
> strictly prohibited and possibly a violation of federal or state
> law and regulations. If you have received this information in
> error, please notify Baylor Health Care System immediately at
> 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor
> Health Care System, its subsidiaries, and affiliates hereby claim
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