Re: Setting ink on tissues

From:Shaun Kay

Could you tell us what pH we are talking about as I have recently had a
problem with the india ink staying on the tissue during processing.  Its
been fine for 15 years and now starts to play up
Mr Shaun Kay
Chief Laboratory Scientist
Davies Campbell deLambert Pathology
2 Leeds Street
NSW 2138
Tel 02 9736 2555
----- Original Message -----
From: "Jennings-Siena, Debbie" 
To: "Charles.Embrey" ; "Linda McDonald"

Sent: Thursday, July 18, 2002 7:09 AM
Subject: RE: Setting ink on tissues

> It is my understanding that it is the pH that sets the India Ink.  I have
been using out of date decal solution and it works great.
> Debbie J. Siena, HT(ASCP)QIHC
> Histopathology Manager
> Baylor University Medical Center
> 3500 Gaston Ave.
> Dallas, TX 75246
> 214-820-2465 vm
> 214-820-4110 fax
> -----Original Message-----
> From: Charles.Embrey []
> Sent: Wednesday, July 17, 2002 11:06 AM
> To: 'Linda McDonald'
> Cc: ''
> Subject: RE: Setting ink on tissues
> Vinegar (dilute GAA) has been use for decades to set dyes in the clothing
> industry.  It is the GAA in the bouin's solution as well as the picric
> that sets the ink so just making dilute GAA is much safer and cost
> effective.  I keep a spray bottle of 5% GAA next to my cutting board to
> spray the specimens.  No mess little waste.
> Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
> Histology Manager
> Carle Clinic
> Urbana, IL
> -----Original Message-----
> From: Linda McDonald []
> Sent: Wednesday, July 17, 2002 10:44 AM
> To:
> Subject: Setting ink on tissues
> Hello!  Question for the netters:  Who or when or how did it come about to
> use a dilute glacial acetic acid solution to help set ink on tissues.
> is used in place of dunking in Bouin's.  Anybody know?
> Thanks,
> Linda McDonald
> Cleveland Clinic Health Systems
> Cleveland, OH
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