HI Katie,

If the brain has been perfused properly it is fixed and can sit in formalin
as long as you want it to unless you are perfusing it for a specific
reason, e.g. ICC

Cheers

Steve


                                                                                                                                               
                    "Crosby,                                                                                                                   
                    Katherine"            To:     "'histonet@pathology.swmed.edu'"                               
                                Subject:     Perfused mouse brain                                                                    
                                                                                                                                               
                    01/08/2002                                                                                                                 
                    15:55                                                                                                                      
                                                                                                                                               
                                                                                                                                               




Hello All,
We have some perfused mouse parts, including brain, and would like to know
how long we should allow them to sit in formalin before processing.  Should
we keep everything cold?
Thanks!
Katie

Katie Crosby
Manager, Immunohistochemistry
Cell Signaling Technology
166B Cummings Center
Beverly, MA  01915

Phone: 978-867-2352
Fax:  978-867-2402
E-mail:  kcrosby@cellsignal.com