Hi Chris
PR: clone 1A6, Novocastra NCL-PGR, at approx. 1 ug Ig/ml (i.e. 1:40);
pretreatment in citrate buffer, pressure cooker.
p16: clone G175-405, Pharmingen 13251A (now: Becton Dickinson, 554070); at 5
ug Ig/ml; pretreatment in citrate buffer, pressure cooker.
Visualization is with a ABC/HRP- DAB system. Hope this helps.

Andi Kappeler
Insititute of Pathology, University of Bern, Switzerland


----- Original Message -----
From: "C Sawrenko" 
To: "HistoNet Server" 
Sent: Tuesday, July 23, 2002 2:24 AM
Subject: PR and p16


> Hello Histonetters,
> We are again in need of your valuable feedback!  What suppliers and clones
> are people using for PR?  How about p16?
> Thanks in advance!
>
> Chris Sawrenko
> Histopathology
> BC Cancer Agency
> Vancouver, BC  Canada
>
>
>