Re: Mouse testes processing
Zinc formalin may also be a good fixative choice for testes.
> Hi Teri,
> We also use Bouin's now, having used formalin for many years. The capsule
> seems to be a barrier and the tubules in the centre do suffer. As an
> alternative to Bouin's we have tried Davidson's, which works well and does
> not have the sectioning problems of Bouin's.
> "Johnson, Teri"
> itute.org> cc:
> Subject: Mouse testes processing
> 30/07/2002 16:21
> Hello all!
> I'm having difficulty with routine processing of mouse testes. I haven't
> been able to preserve the intertubular connective tissue. I've tried
> bouins fixative and it's well preserved. It mostly disappears with 4%
> paraformaldehyde and 10% neutral buffered formalin. The simple answer is
> to use bouins, but what I'd really like to do is figure out how to preserve
> it with routine fixation/processing.
> My processor is set up as follows:
> 70% alcohol
> Prosoft x5 stations
> ProPar x3 stations
> paraffin x4 stations
> I will post a picture to the histonet.org site and will send another email
> with the path when it's posted.
> Also, for those of you using Davidson's fixative on mouse tissues, have
> there been many problems with subsequent immunostaining?
> Thanks for your help,
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri 64110
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