Zinc formalin may also be a good fixative choice for testes.
Patsy

Stephen.Eyres@sanofi-synthelabo.com wrote:

> Hi Teri,
>
> We also use Bouin's now, having used formalin for many years. The capsule
> seems to be a barrier and the tubules in the centre do suffer. As an
> alternative to Bouin's we have tried Davidson's, which works well and does
> not have the sectioning problems of Bouin's.
> Cheers
> Steve
>
>
>                     "Johnson, Teri"
>                     
>                     itute.org>               cc:
>                                              Subject:     Mouse testes processing
>                     30/07/2002 16:21
>
>
>
> Hello all!
>
> I'm having difficulty with routine processing of mouse testes.  I haven't
> been able to preserve the intertubular connective tissue.  I've tried
> bouins fixative and it's well preserved.  It mostly disappears with 4%
> paraformaldehyde and 10% neutral buffered formalin.  The simple answer is
> to use bouins, but what I'd really like to do is figure out how to preserve
> it with routine fixation/processing.
>
> My processor is set up as follows:
> 70% alcohol
> Prosoft x5 stations
> ProPar x3 stations
> paraffin x4 stations
>
> I will post a picture to the histonet.org site and will send another email
> with the path when it's posted.
>
> Also, for those of you using Davidson's fixative on mouse tissues, have
> there been many problems with subsequent immunostaining?
>
> Thanks for your help,
>
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110
> tjj@stowers-institute.org