Re: Mouse testes processing

Hi Teri,

We also use Bouin's now, having used formalin for many years. The capsule
seems to be a barrier and the tubules in the centre do suffer. As an
alternative to Bouin's we have tried Davidson's, which works well and does
not have the sectioning problems of Bouin's.

                    "Johnson, Teri"                                                                                                               
          >               cc:                                                                                                  
                                             Subject:     Mouse testes processing                                                                 
                    30/07/2002 16:21                                                                                                              

Hello all!

I'm having difficulty with routine processing of mouse testes.  I haven't
been able to preserve the intertubular connective tissue.  I've tried
bouins fixative and it's well preserved.  It mostly disappears with 4%
paraformaldehyde and 10% neutral buffered formalin.  The simple answer is
to use bouins, but what I'd really like to do is figure out how to preserve
it with routine fixation/processing.

My processor is set up as follows:
70% alcohol
Prosoft x5 stations
ProPar x3 stations
paraffin x4 stations

I will post a picture to the site and will send another email
with the path when it's posted.

Also, for those of you using Davidson's fixative on mouse tissues, have
there been many problems with subsequent immunostaining?

Thanks for your help,

Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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