Re: LCM

From:

richard hazelton

Dear Anne,

I suggest that you try using poly-L-lysine coated slides.

Whilst I haven't used this for bone, it was useful to keep frozen sections of mouse eyes adhered, without interfering with lcm.

hooroo, richard

----- Original Message -----

From: Anne Undersander

To: HistoNet Server

Sent: Saturday, August 03, 2002 2:06 AM

Subject: LCM

Has anyone had experience doing LCM on frozen sections of bone/catilage? Our problem is when we use charged slides we can't pick up cells but, if we use non-charged slides the sections, of course, fall off. Any help would be appreciated!

Thanks.

Anne Undersander HT(ASCP)
Vet. Med./Animal Science
University of Minnesota

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