Re: Iron Stains
Sounds like the iron is being lost. What's in B-5? Does it contain acid to
help part decalcify?
Subject: Iron Stains
I seem to have a problem seeing Iron in my Bone Marrow clot sections. My
control always works- it is a purchased positive control that is formalim
fixed. I use B-5 to fix my clots and the bone bx. The direct asperate
smear shows iron most of the time and that is fixed with methyl alcohol.
this smear is air dried after the iron stain water rinse. My clot sections
are brought through alcohols and xylene and then coverslipped. Am i
washing out my iron in the Alcohols? Is there anyone who uses B-5 for bone
marrow fixative? What is the secret? please help me.
Warren General Hospital
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