Re: Iron Stains

I seem to have a problem seeing Iron in my Bone Marrow clot sections. My control always works- it is a purchased positive control that is formalim fixed. I use B-5 to fix my clots and the bone bx. The direct asperate smear shows iron most of the time and that is fixed with methyl alcohol. this smear is air dried after the iron stain water rinse. My clot sections are brought through alcohols and xylene and then coverslipped. Am i washing out my iron in the Alcohols? Is there anyone who uses B-5 for bone marrow fixative? What is the secret? please help me.