Re: Iron Stains
You might wish to consider that B5 is an acidic
fixative, this could well be removing the hemosiderin.
Bryan R. Hewlett
Hamilton Regional Laboratory Medicine
----- Original Message -----
Sent: Wednesday, July 24, 2002 4:39
Subject: Iron Stains
I seem to have a problem seeing Iron in my Bone
Marrow clot sections. My control always works- it is a purchased positive
control that is formalim fixed. I use B-5 to fix my clots and the bone bx. The
direct asperate smear shows iron most of the time and that is fixed with
methyl alcohol. this smear is air dried after the iron stain water rinse. My
clot sections are brought through alcohols and xylene and then coverslipped.
Am i washing out my iron in the Alcohols? Is there anyone who uses B-5 for
bone marrow fixative? What is the secret? please help me.
Warren General Hospital
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