Re: Iron Stains

From:Bryan Hewlett

You might wish to consider that B5 is an acidic fixative, this could well be removing the hemosiderin.
Bryan R. Hewlett
Technical Specialist
Anatomical Pathology
Hamilton Regional Laboratory Medicine Program
Ontario, Canada
----- Original Message -----
From: Tim Walter
To: HistoNet Server
Sent: Wednesday, July 24, 2002 4:39 PM
Subject: Iron Stains

I seem to have a problem seeing Iron in my Bone Marrow clot sections. My control always works- it is a purchased positive control that is formalim fixed. I use B-5 to fix my clots and the bone bx. The direct asperate smear shows iron most of the time and that is fixed with methyl alcohol. this smear is air dried after the iron stain water rinse. My clot sections are brought through alcohols and xylene and then coverslipped. Am i washing out my iron in the Alcohols? Is there anyone who uses B-5 for bone marrow fixative? What is the secret? please help me.
                            Joyce Walter
                            Warren General Hospital
Warren, Pa

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