Re: Formalin Pigment

From:Tony Henwood

Re: Formalin Pigment

Try the following web site - suggests phenol

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Jane Hosmer []
Sent: Saturday, 3 August 2002 5:49
To: HistoNet Server
Subject: re: subscribe

I am a new subscriber and need to know how to ask a question. Please E-mail
me as soon as possible. We are having a problem in that we use
ammonia-alcohol to remove formalin-pigment as a result of fixing our tissue
in 70% alcoholic formalin.  But with our new Sakura Stainer, we have mega
fumes from the ammonia.  Does anyone else have this problem?   Do you have
another procedure for removing formalin pigment without using the
ammonia-alcohol or picric acid-alcohol?  Thanks for your help.

Jane Hosmer,HTL and Marilyn Shackelford,HTL
Comparative Pathology Laboratory
UAB at Birmingham, Ala

At 01:20 PM 7/23/02 -0500, you wrote:
>Your address has been added to the addresses that comprise this  Listserv
>Welcome to HISTONET. This is an electronic mailing list for the exchange of
>information pertaining to histotechnology and related fields.
>It contains useful information about how to use the list and what to do if
>experience problems. It also includes some basic rules for email etiquette
>(Netiquette) which will be helpful to those who are new to this form of
>A list server is a computer that runs software which will receive incoming
>electronic mail (email) messages and reroute them automatically to
everyone on
>the subscriber list. Email uses the vast expanse of the Internet to allow
>almost instantaneous communication between networked computers around the
>world. Our system uses the LISTSTAR software from Quarterdeck Corporation
>(California) and can currently send about 30 messages a minute.  With the
>present number of subscribers, we are processing about 10,000 outbound
>messages a day.
>Anyone interested in research or clinical applications of histology,
>immunohistochemistry, in-situ hybridization pathology, and electron
>may find Histonet informative and useful. Currently, there are more than 850
>subscribers from all over the world. Subscribers include hospital employees
>from major urban centers and obscure remote locales, university researchers,
>botanists and the employees of commercial laboratories, government agencies,
>veterinary facilities and a wide variety of commercial industrial ventures.
>The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using
>hardware and software owned by the University of Texas Southwestern Medical
>School, Department of Pathology in Dallas, Texas. If you have any
questions or
>problems with Histonet please contact Linda Margraf at
>This server, unlike many systems, uses ONLY ONE ADDRESS to send commands to
>the computer and to post messages. The server will recognize commands sent in
>the SUBJECT line of the message and only when they are spelled exactly as
>listed below. Anything not identified as a command will be circulated to
>EVERYONE on the list.
>The following is a list of commands the server recognizes:
> Your address will be added to the list of subscribers. You will then be able
>to send messages to this list that will be forwarded to all other list
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>subscribe digest
> Your address will be added to the list of subscribers who receive a digest
>instead of each forwarded message. A digest is a compilation of all the
>messages received in a 24 hour period. It is sent to the digest subscribers
>every night after midnight. Digest subscribers can post and respond to
>messages the same as "real-time" subscribers.
> A list of available digests will be returned to you. Histonet stores old
>messages as daily digests for approximately three  months. To read previous
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> Your address will be removed from the list of subscribers.
> You will no longer be able to send messages to the members
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> A list of the commands recognized by the server will be   returned to you.
>You may post any questions you wish pertaining to histology, pathology,
>in-situ hybridization, immunohistochemistry etc. Equipment and reagent
>evaluations, laboratory management issues, government regulations, and job
>opportunities are all appropriate topics. The University asks that we
>the use of its hardware and software to business purposes only (occasional
>jokes do slip through but PLEASE use restraint). Vendors and those with
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>we ask that blatant advertisements be avoided at all times. It is fine to
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> if you are not sure about the appropriateness
>if a message you wish to post.
>It is most helpful to the list members if you post your responses to queries
>to everyone on the list and not just as a personal reply to the person asking
>the question. That way duplicate messages are minimized and we all learn from
>each other's comments.
>Likewise, if you post a question and get a number of responses back directly
>to you, it is helpful to everyone if you could send out a summary of the
>replies you got to Histonet.
>Please avoid abbreviations unless they are explained in your message. For
>example: immunohistochemistry (IHC). This list circulates to a wide
variety of
>individuals and what seems obvious to you may have no meaning on the other
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>Please sign your letter and include your institution or affiliation and
>location. Not all email systems have headers which identify the sender.
>Do use the subject line to indicate the topic of your message.
>DON'T USE ONLY CAPITAL LETTERS -it is considered shouting.
>Please send questions and problems about the list directly to Linda
Margraf at
> and don't circulate them to the >850
>subscribers on the list. Be careful when sending commands to the server to
>the command in the SUBJECT LINE and spell it correctly.
>Please do not send images as attachments with your message. We can now post
>images at our web site ( To have an image posted
>send it to Herb Hagler at

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