Re: Fluorogold

From:Gayle Callis

Hi Cheryl,

We put frozens on Plus charge for IFA technics, frozens and polysine should
work.  There are also Plus gold that help with acetone fixed sections, bit
more expensive, get some samples from Erie Scientific, supposed to have
superior holding power, according to directions, acetone fixed but some
people use them for MOHS, and even aldehyde fixation.  Not sure why Erie
says for acetone fixation??  worth a try. 

You can also let the sections dry longer over 16 mesh silica gel, that may
help too - overnight.  

At 07:43 AM 7/22/02 -0500, you wrote:
>Good morning Histonetters - I have a question from a graduate student and 
>having never done this technqiue I am at a loss.  The graduate is trying 
>to do a fluorogold technique on ganglia.  She is cutting frozen 
>sections.  The sections are terrible (I will help her there), but they 
>consistently wash off.  
>     Question:  Should the tissues be put on coated slides, Probe-On 
>slides, charged slides, etc. to help the tissue stick or will that 
>interfere with the fluorgold deposition?  
>     Any help you can offer will be greatly appreciated.  Cheryl
>Cheryl Crowder, BA, HTL(ASCP)
>Chief Technologist
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Baton Rouge, LA  70803
>(225) 578-9784
>FAX  (225) 578-9720
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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