Lawrence Brett wrote:

> Dako's liquid DAB is good but it's not a magic formula.  The following
> should be at least as sensitive but won't keep for as long (I prepare it
> fresh each day).
>
> 0.005M Tris-HCL 100mLs
> DAB 10mg
> Imidazole 6mg
> Tween 20  0.1mL
> 6% H2O2 0.4mLs
>
> If there are any budding chemists out there who can help with the shelf-life
> I'd be interested too. I'm currently experimenting with preservatives such
> as sodium sulphite and metabisulphite.
>
> Good luck
>
> --
> Lawrence Brett
> Immunocytochemistry Lab.
> Pathology Dept.
> Western General Hospital
> Crewe Road South
> Edinburgh  EH4 2XU
> Scotland. UK
> Tel: 0131 537 2148
> email: lauriebrett@bigfoot.com