Re:DAB liquid

From:Lawrence Brett

Dako's liquid DAB is good but it's not a magic formula.  The following
should be at least as sensitive but won't keep for as long (I prepare it
fresh each day).

0.005M Tris-HCL 100mLs
DAB 10mg
Imidazole 6mg
Tween 20  0.1mL
6% H2O2 0.4mLs

If there are any budding chemists out there who can help with the shelf-life
I'd be interested too. I'm currently experimenting with preservatives such
as sodium sulphite and metabisulphite.

Good luck

Lawrence Brett
Immunocytochemistry Lab.
Pathology Dept.
Western General Hospital
Crewe Road South
Edinburgh  EH4 2XU
Scotland. UK
Tel: 0131 537 2148

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