Dako's liquid DAB is good but it's not a magic formula. The following
should be at least as sensitive but won't keep for as long (I prepare it
fresh each day).
0.005M Tris-HCL 100mLs
Tween 20 0.1mL
6% H2O2 0.4mLs
If there are any budding chemists out there who can help with the shelf-life
I'd be interested too. I'm currently experimenting with preservatives such
as sodium sulphite and metabisulphite.
Western General Hospital
Crewe Road South
Edinburgh EH4 2XU
Tel: 0131 537 2148
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