Re: Congo Red

Hi Jennifer,

Amyloid comes in 2 main forms based upon the protein forming the fibrils.
Type AA - from serum protein A and type AL - immunoglobulin light chains.
AA type does not stain and is not birefringent after pre-treatment with
potassium permanganate. The reference is Krishman, J et al, Am J Clin
Pathol 100:135-144, 1993

                    SCHUMACHER           To:                                                                 
                               Subject:     Congo Red                                                                               

We have recently been asked to do a congo red with and without potassium
permanganate treatment.  Does anyone have a procedure for the
length/temperature/percentage for this pre-treatment?  What does this
pre-treatment do (ie. is a normal amyloid control sufficient)? Thanks for
your help.

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