We do the B5 than the formalin also.No problems unless the node was to thick
to start with.
----- Original Message -----
From: "Johnston, Kathy" 
To: "'Sharon Cassar'" ; "HistoNet Server"

Sent: Friday, July 26, 2002 2:44 PM
Subject: RE: B5


> Ours also go through formalin.  I don't think your nodes are properly
fixing
> and therefore are remaining soft.  All our B5 tissues are done with this
> (i.e. nodes, bone marrows, kidneys).
>
> Any other suggestions 'Net?
>
> Kathy
>
> -----Original Message-----
> From: Sharon Cassar [mailto:smcassar@waldonet.net.mt]
> Sent: Friday, July 26, 2002 11:15 AM
> To: HistoNet Server
> Subject: B5
>
>
> Hello
>
> We are using B5 fixative for lymph nodes.  After fixation in B5 for 3 hrs,
> the sections are put in 70% alcohol and then overnight processing,
starting
> from 95% ethanol.  However when cutting, the tissue is very soft and thick
> sections are being produced when compared to formalin fixed lymph node
> sections.  Any suggestions on the schedule to process overnight.  Our
> schedule is as follows
>
> B5 fixative        3hrs
> 70%   ETOH    5 hrs
> 95%   ETOH    2 hrs
> !00%  ETOH    1.30 hr
> 100% ETOH    1.30 hr
> 100% ETOH    1 hr
> Chloroform       1 hr
> Chloroform       1 hr
> Chloroform       1 hr
> Paraffin Wax    1.30 hr
> Paraffin Wax    1hr
>
> Thank you
> Sharon Cassar  MLS
> St. Lukes Hospital
> Malta
>
>
>
>