RE: xylene problems

From:Bruce Gapinski

1) I wouldn't cover them from alcohol.
2) I don't think what you have is xylene. It will not turn slides (tissue)
black. Get a fresh bottle of xylene. Maybe what you have is clear waste?
Bruce Gapinski HT(ASCP)

-----Original Message-----
From: Rafael Badalyan []
Sent: Tuesday, July 16, 2002 12:16 PM
Subject: xylene problems

Dear Histonetters, 
I would like to ask you a question about clearing the slides. I am staining
them with Masson trichrome and when I try to clear colored slides wtih
xylene (aftre alcohole) the colors are becoming very dark (even black) . 
Can I do not clear them with xylene, but mount after  dehydrating in
Please help, I am first time in staining. 
Thanks in advance , 

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