RE: xylene problems
1) I wouldn't cover them from alcohol.
2) I don't think what you have is xylene. It will not turn slides (tissue)
black. Get a fresh bottle of xylene. Maybe what you have is clear waste?
Bruce Gapinski HT(ASCP)
From: Rafael Badalyan [mailto:firstname.lastname@example.org]
Sent: Tuesday, July 16, 2002 12:16 PM
Subject: xylene problems
I would like to ask you a question about clearing the slides. I am staining
them with Masson trichrome and when I try to clear colored slides wtih
xylene (aftre alcohole) the colors are becoming very dark (even black) .
Can I do not clear them with xylene, but mount after dehydrating in
Please help, I am first time in staining.
Thanks in advance ,
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