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-----Original Message-----
From: Ronnie_Houston@bshsi.com
[mailto:Ronnie_Houston@bshsi.com]
Sent: Thursday, July 18, 2002 5:21 AM
To: histonet@pathology.swmed.edu;
antje.marcantonio@pharma.novartis.com
Subject: Re: zinc fixative and sma
We routinely use zinc formalin for all tissues, and
don't have a problem
with background staining, quite the contrary; clean
background and crisp
staining.
What detection system are you using?
Ronnie Houston
Regional Histology Operations Manager
Bon Secours Health Partners Laboratories
5801 Bremo Road
Richmond, VA 23226
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Subject: zinc fixative and sma
Author: antje.marcantonio@pharma.novartis.com at BSHSIBTW
Date: 7/18/02 5:09 AM
Hello Histonetters
it has been discussed many times about zinc fixative
(=formalin-free) ->
histonet archive .We like to use it to stain surface
markers,
e.g. CD3,
CD4, CD8, CD20 in monkey tissue, since in formalin
fixed
paraffin embedded
tissue it is much more difficult to get an acceptable
staining
intensity
without background.
But now we would like to stain for smooth muscle
actin in zinc
fixed
material. So far the results are disappointing
because of high
background.
In FFPE the staining looks nice and clean. Any
explanation for that ?
Thank you for your help.
Antje Marcantonio
Novartis Pharma AG, Basel CH
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