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From:Bruce Gapinski

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		-----Original Message-----
		From:	Ronnie_Houston@bshsi.com
[mailto:Ronnie_Houston@bshsi.com]
		Sent:	Thursday, July 18, 2002 5:21 AM
		To:	histonet@pathology.swmed.edu;
antje.marcantonio@pharma.novartis.com
		Subject:	Re: zinc fixative and sma




		     We routinely use zinc formalin for all tissues, and
don't have a problem
		     with background staining, quite the contrary; clean
background and crisp
		     staining.
		     What detection system are you using?

		     Ronnie Houston
		     Regional Histology Operations Manager
		     Bon Secours Health Partners Laboratories
		     5801 Bremo Road
		     Richmond, VA 23226


		______________________________ Reply Separator
_________________________________
		Subject: zinc fixative and sma
		Author:  antje.marcantonio@pharma.novartis.com at BSHSIBTW
		Date:    7/18/02 5:09 AM




		       Hello Histonetters

		       it has been discussed many times about zinc fixative
		       (=formalin-free) ->
		       histonet archive .We like to use it to stain surface
markers,
		       e.g. CD3,
		       CD4, CD8, CD20 in monkey tissue, since in formalin
fixed
		       paraffin embedded
		       tissue it is much more difficult to get an acceptable
staining
		       intensity
		       without background.
		       But now we would like to stain for smooth muscle
actin in zinc
		       fixed
		       material. So far the results are disappointing
because of high
		       background.
		       In FFPE the staining looks nice and clean. Any
explanation for that ?
		       Thank you for your help.

		       Antje Marcantonio
		       Novartis Pharma AG, Basel CH



		




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