RE: sterilization of instruments

From:Robert Geske

Amos the "sterilization" is really to drive off any residual DEPC after it
has done its thing.

rob

-----Original Message-----
From: Amos Brooks [mailto:amosbrooks@comcast.net]
Sent: Tuesday, July 30, 2002 4:52 PM
To: histonet@pathology.swmed.edu
Subject: Re: sterilization of instruments


Hi,
    "Autoclaved DEPC water"? ... Pardon the almost inevitably silly question
but why autoclave the DEPC water?
Amos Brooks

----- Original Message -----
From: 
To: 
Sent: Tuesday, July 30, 2002 4:53 PM
Subject: Re: sterilization of instruments


>
> I had a feeling that is where you were headed with the question. Rnase
Away
> is dissection friendly..... I think we get it from Fisher but not sure.
> Molecular BioProducts www.mbpinc.com we dip, wipe and rinse in
> autoclaved/DEPC water wipe again and you are ready to go and no open
flame.
> Let me know if you need more of a protocol. but it works for us and has
for
> quite some time.
> anita
>
>
>
>                     Kathleen
>                     Cormier              To:
histonet@pathology.swmed.edu
>                                          DU>                  Subject:     sterilization of
instruments
>
>                     07/30/2002
>                     12:24 PM
>
>
>
>
>
>
> Hello!
>
> In response to a few replies, we need to sterilize in between samples and
> or animals because we are taking multiple sterile samples for PCR and or
> RNA and or rapid urease, each from the same animal. Disposable instruments
> have been deemed too costly, and moving the "flame" away from the ETOH
> while simple still does not seem to work for some people. Environmental
> Health and Safety would like us to eliminate the open flame if possible.
>
> Thanks!!
>
> Kathy Cormier
> DCM-MIT
>
>
>
>
>
>
>
>



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