I have stained deparaffinized sections with NBT/BCIP using a modification of
the method in Bancroft and Cook (1994, pg
 300). Also, anti-Osteocalcin from Zymed # 33-5700 and anti-Cbfa1 from Alpha
Diagnostic # CBFA11-A work well in formalin fixed paraffin embedded bone and
in cultured cells.Donna Montague, M.S.
Research Associate
University of Arkansas for Medical Sciences
Center for Orthopaedic Research
Departments of Orthopaedic Surgery and 
Physiology & Biophysics


-----Original Message-----
From: Jan Shivers [mailto:shive003@maroon.tc.umn.edu]
Sent: Wednesday, July 24, 2002 12:10 PM
To: HistoNet Server
Subject: osteoblast Ab


Does anyone have an antibody source for an osteoblast marker?  And, have you
had any experience using it on non-human mammals with success?

Jan Shivers
Scientist, IHC
Univ. of Minn. Veterinary Diagnostic Lab