RE: frozen sections fixed in formalin
We usually prepare a saline solution of 20% sucrose. We left the tissues for
2 days in that solution and we freeze with N-exane.
The results are wondeful!
> -----Original Message-----
> From: CRME (Criss Meligro) [SMTP:email@example.com]
> Sent: giovedý 25 luglio 2002 20:57
> To: 'firstname.lastname@example.org'
> Subject: frozen sections fixed in formalin
> We have just received some tissue for Oil Red O that has been fixed in
> formalin... I know that the water must be exchanged with a sucrose
> but don't remember the concentrations that work best.... currently we have
> it in 10%. Any suggestions appreciated.
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