We usually prepare a saline solution of 20% sucrose. We left the tissues for
2 days in that solution and we freeze with N-exane. 
The results are wondeful!

Regards, 
Silvia
> -----Original Message-----
> From:	CRME (Criss Meligro) [SMTP:meligroc@zgi.com]
> Sent:	giovedì 25 luglio 2002 20:57
> To:	'histonet@pathology.swmed.edu'
> Subject:	frozen sections fixed in formalin
> 
> We have just received some tissue for Oil Red O that has been fixed in
> formalin... I know that the water must be exchanged with a sucrose
> solution
> but don't remember the concentrations that work best.... currently we have
> it in 10%.  Any suggestions appreciated.