RE: frozen sections fixed in formalin

From:"Pasquetto, Silvia"

We usually prepare a saline solution of 20% sucrose. We left the tissues for
2 days in that solution and we freeze with N-exane. 
The results are wondeful!

> -----Original Message-----
> From:	CRME (Criss Meligro) []
> Sent:	giovedý 25 luglio 2002 20:57
> To:	''
> Subject:	frozen sections fixed in formalin
> We have just received some tissue for Oil Red O that has been fixed in
> formalin... I know that the water must be exchanged with a sucrose
> solution
> but don't remember the concentrations that work best.... currently we have
> it in 10%.  Any suggestions appreciated.

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