You only need to block for biotin if your tissue has endogenous biotin.  I
will do biotin block if my neg control has a lot of background.  Our lab
does not routinely block for biotin.

Robin Stocks

-----Original Message-----
From: Wendy England [mailto:weneng@hotmail.com]
Sent: July 26, 2002 11:24 AM
To: histonet@pathology.swmed.edu
Subject: biotin blocking


Dear histonetters,

Should I do biotin blocking on cell sample when I use avidin-biotin method?
Thanks in advance!
Wendy



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