RE: Sterilization of instruments

From:"Charles W. Scouten, Ph.D."

Try the hot bead sterilization, you will love it.  Beads on the surface
do not get very hot, the heat is carried away by air flow faster than it
arrives through all the glass.  But just below the surface is blazing
hot glass.  It sears the surface of each instrument, before the inside
of the metal is fully hot, the surface is thoroughly sterile.  Thus,
both heating and cooling take a few seconds, and ready to go again.  As
effective as autoclave (except it doesn't sterilize the handles), but
much much faster.

See the following URL:


Charles W.  Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300  
FAX  314 522 0277

-----Original Message-----
From: Susan Bell [] 
Sent: Tuesday, July 30, 2002 12:59 PM
To: Kathleen Cormier;
Subject: Re: Sterilization of instruments


We use autoclaved instruments, a new set for each animal.  Can end up 
being a lot of instruments (often more than 20), but we are a 
research facility, and therefore we have to be sure there is not any 
cross contamination between animals.

I hope this helps.

Susan Bell

At 12:02 PM -0400 7/30/02, Kathleen Cormier wrote:
>Hello all!
>What is the preferred method of sterilizing instruments during 
>necropsy of multiple animals? We may do upwards of 20 animals during 
>a session. What we do is in between animals or samples is to dip the 
>instruments in 70% ETOH then "flame" them, allow to cool off for 20 
>seconds them proceed with dissection. After one too many beaker 
>fires we are being asked to change our procedure. One suggestion is 
>heated bead sterilization in between samples/ animals another is to 
>order 30 additional sets of instruments and autoclave the sets prior 
>to use. Any suggestions?
>Kathy Cormier
>Histology Supervisor
>Div Comparative Med


Susan Bell
Research Assistant
Targeted Genetics Corporation
1100 Olive Way  Suite 100
Seattle WA  98101
Phone:  206-521-4830
Fax:  206-223-0288

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