RE: Staining for Fat Laden Macrophages in Bronchio-Alveolar lavages
make cytospins, fix in 10% NBF and then do an oil red O when looking for fat
Can you help? In my laboratory we are currently reviewing
our procedure for the fixing of BAL preparations for fat laden macrophages.
Does anybody else stain for fat laden macrophages? What preparation techniques
and fixatives are you using?
Thanks in advance
Alder Hey Hospital
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