RE: Staining for Fat Laden Macrophages in Bronchio-Alveolar lavag es

From:Tony Henwood

We smear or cytospin (depending on the volume &/or viscosity of the fluid) the material on adhesive coated slides, air dry and then stain using the standard Oil Red O technique. If you like you can fix the smears in formalin fumes (Place slides in a coplin jar, label side down, add 1-2ml contentrated formalin, cover and leave overnight), and then stain as above. Counterstain the smears with haematoxylin so the cytologists can identify the macrophages from other elements that might contain fat and your done.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: David Muskett []
Sent: Friday, 2 August 2002 5:41
To: histonet
Subject: Staining for Fat Laden Macrophages in Bronchio-Alveolar lavages

Dear Histonetters
Can you help? In my laboratory we are currently reviewing our procedure for the fixing of BAL preparations for fat laden macrophages. Does anybody else stain for fat laden macrophages? What preparation techniques and fixatives are you using?
Thanks in advance
David Muskett
Histology Lab
Alder Hey Hospital
Liverpool, UK

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