RE: Stain for Alzheimer's disease
| From: | Andrew.Shand@north-bristol.swest.nhs.uk |
Deb
Remember that all neuropath silvers are, to say the least, capricious (bitter experience).
They can be used to demonstrate anything by varying time, temp, pH etc.
Use the method as a guide but be prepared to vary to suit local
conditions. If possible get a method from a lab with the same water
hardness.
Another avenue worth exploring is immuno. It's
easier and produces good results. Antibodies worth looking at include
PHF-Tau-AT8, Alzheimer Precursor Protein A4, Beta Amyloid and Beta Amyloid
Peptide.
I am looking for a stain to show the
neurofibrillary changes found in Alzheimer's disease. A couple of
findings from our pathologist was the Cross Modified Palmgren stain, with no
reference on how to prepare the stain and the Bielschowski's technique.
Anyone have any suggestions?
Thanks,
Deb Croegaert
Metro Labs
Davenport<
Iowa
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