RE: Spray Fixative for Cell preps
Don't
use hair spray. Its use in cytology was discredited long ago. The
composition is usually undefined. If by chance you're using a hair spray
that produces results you like, the manufacturer can change the composition
without your knowing it. The new formula may produce different
results.
To
make spray fixative that equals commercially available spray fixatives, simply
dissolve 20 gm Carbowax 1450 in 95% ethanol up to 1,000 mL. Denatured
alcohol works equally well. You may want to dilute it to 95%
concentration, but it won't make a visibly detectable difference in
cytomorphology. (Denatured alcohol is 9 parts ethanol, 0.5 parts
isopropanol, and 0.5 parts methanol.)
Gary
Gill
We have a client that is preparing cytospin preps
for a research project without a lot of funding. Does anyone out there
have a formula for making up spray fixative from scratch? Have inspected
the bottle that we purchase commercially and have all of the reagents needed
but can't locate a protocol. Thanks in advance for any
assistance.
Amy S.Porter, HT(ASCP)
Michigan State
University
College of Human Medicine
portera203@yahoo.com
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