Use a low pH, such as an weak acid.  That is why decal solution works well.
Debbie J. Siena, HT(ASCP)QIHC
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 vm
214-820-4110 fax
debbiesi@baylordallas.edu


-----Original Message-----
From: Shaun Kay [mailto:skay@dcdel.com.au]
Sent: Wednesday, July 17, 2002 6:59 PM
To: histonet@pathology.swmed.edu
Subject: Re: Setting ink on tissues


Could you tell us what pH we are talking about as I have recently had a
problem with the india ink staying on the tissue during processing.  Its
been fine for 15 years and now starts to play up
Mr Shaun Kay
Chief Laboratory Scientist
Davies Campbell deLambert Pathology
2 Leeds Street
Rhodes
NSW 2138
Australia
Tel 02 9736 2555
or skay@dcdel.com.au
----- Original Message -----
From: "Jennings-Siena, Debbie" 
To: "Charles.Embrey" ; "Linda McDonald"

Cc: 
Sent: Thursday, July 18, 2002 7:09 AM
Subject: RE: Setting ink on tissues


>
> It is my understanding that it is the pH that sets the India Ink.  I have
been using out of date decal solution and it works great.
> Debbie J. Siena, HT(ASCP)QIHC
> Histopathology Manager
> Baylor University Medical Center
> 3500 Gaston Ave.
> Dallas, TX 75246
> 214-820-2465 vm
> 214-820-4110 fax
> debbiesi@baylordallas.edu
>
>
> -----Original Message-----
> From: Charles.Embrey [mailto:Charles.Embrey@carle.com]
> Sent: Wednesday, July 17, 2002 11:06 AM
> To: 'Linda McDonald'
> Cc: 'histonet@pathology.swmed.edu'
> Subject: RE: Setting ink on tissues
>
>
> Vinegar (dilute GAA) has been use for decades to set dyes in the clothing
> industry.  It is the GAA in the bouin's solution as well as the picric
acid
> that sets the ink so just making dilute GAA is much safer and cost
> effective.  I keep a spray bottle of 5% GAA next to my cutting board to
> spray the specimens.  No mess little waste.
> Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
> Histology Manager
> Carle Clinic
> Urbana, IL
>
>
> -----Original Message-----
> From: Linda McDonald [mailto:mcdonal1@ccf.org]
> Sent: Wednesday, July 17, 2002 10:44 AM
> To: histonet@pathology.swmed.edu
> Subject: Setting ink on tissues
>
>
> Hello!  Question for the netters:  Who or when or how did it come about to
> use a dilute glacial acetic acid solution to help set ink on tissues.
This
> is used in place of dunking in Bouin's.  Anybody know?
> Thanks,
> Linda McDonald
> Cleveland Clinic Health Systems
> Cleveland, OH
>
>
>
>
>
>
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This e-mail, facsimile, or letter and any files or attachments 
transmitted with it contains information that is confidential and 
privileged. This information is intended only for the use of the 
individual(s) and entity(ies) to whom it is addressed. If you are 
the intended recipient, further disclosures are prohibited without 
proper authorization. If you are not the intended recipient, any 
disclosure, copying, printing, or use of this information is 
strictly prohibited and possibly a violation of federal or state 
law and regulations. If you have received this information in 
error, please notify Baylor Health Care System immediately at 
1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor 
Health Care System, its subsidiaries, and affiliates hereby claim 
all applicable privileges related to this information.