It is my understanding that it is the pH that sets the India Ink.  I have been using out of date decal solution and it works great.
Debbie J. Siena, HT(ASCP)QIHC
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 vm
214-820-4110 fax
debbiesi@baylordallas.edu


-----Original Message-----
From: Charles.Embrey [mailto:Charles.Embrey@carle.com]
Sent: Wednesday, July 17, 2002 11:06 AM
To: 'Linda McDonald'
Cc: 'histonet@pathology.swmed.edu'
Subject: RE: Setting ink on tissues


Vinegar (dilute GAA) has been use for decades to set dyes in the clothing
industry.  It is the GAA in the bouin's solution as well as the picric acid
that sets the ink so just making dilute GAA is much safer and cost
effective.  I keep a spray bottle of 5% GAA next to my cutting board to
spray the specimens.  No mess little waste.
Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
Histology Manager
Carle Clinic
Urbana, IL


-----Original Message-----
From: Linda McDonald [mailto:mcdonal1@ccf.org]
Sent: Wednesday, July 17, 2002 10:44 AM
To: histonet@pathology.swmed.edu
Subject: Setting ink on tissues


Hello!  Question for the netters:  Who or when or how did it come about to
use a dilute glacial acetic acid solution to help set ink on tissues.  This
is used in place of dunking in Bouin's.  Anybody know?  
Thanks,
Linda McDonald
Cleveland Clinic Health Systems
Cleveland, OH






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