RE: Setting ink on tissues


Vinegar (dilute GAA) has been use for decades to set dyes in the clothing
industry.  It is the GAA in the bouin's solution as well as the picric acid
that sets the ink so just making dilute GAA is much safer and cost
effective.  I keep a spray bottle of 5% GAA next to my cutting board to
spray the specimens.  No mess little waste.
Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
Histology Manager
Carle Clinic
Urbana, IL

-----Original Message-----
From: Linda McDonald []
Sent: Wednesday, July 17, 2002 10:44 AM
Subject: Setting ink on tissues

Hello!  Question for the netters:  Who or when or how did it come about to
use a dilute glacial acetic acid solution to help set ink on tissues.  This
is used in place of dunking in Bouin's.  Anybody know?  
Linda McDonald
Cleveland Clinic Health Systems
Cleveland, OH

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