RE: Mouse testes processing

From:"Edwards, R.E."

  We  process  the  Bouins  testes  alongside  the  10%NBF fixed  ones,  works  for  us!!...........
                                                                                                      Richard Edwards
                                                                                                   MRC TOXICOLOGY UNIT....U.K....

-----Original Message-----
From: Pasquetto, Silvia []
Sent: 31 July 2002 16:25
To: 'Johnson, Teri'
Cc: Histonet chat (E-mail)
Subject: RE: Mouse testes processing

We use ROUTINELY Bouin for Testes, epidydimes and eyes (even if we would try
Davidson for eyes) and formalin for all other tissues.

> -----Original Message-----
> From:	Johnson, Teri []
> Sent:	marted́ 30 luglio 2002 17:21
> To:	Histonet (E-mail)
> Subject:	Mouse testes processing
> Hello all!
> I'm having difficulty with routine processing of mouse testes.  I haven't
> been able to preserve the intertubular connective tissue.  I've tried
> bouins fixative and it's well preserved.  It mostly disappears with 4%
> paraformaldehyde and 10% neutral buffered formalin.  The simple answer is
> to use bouins, but what I'd really like to do is figure out how to
> preserve it with routine fixation/processing.
> My processor is set up as follows:
> 70% alcohol
> Prosoft x5 stations
> ProPar x3 stations
> paraffin x4 stations
> I will post a picture to the site and will send another email
> with the path when it's posted.
> Also, for those of you using Davidson's fixative on mouse tissues, have
> there been many problems with subsequent immunostaining?
> Thanks for your help,
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110

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