RE: MGG and blue artifact

From:Tony Henwood

Some glass slides will also stain non-specifically with the dyes giving a blue colouration. Try staining a clean slide.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: []
Sent: Thursday, 18 July 2002 8:15
Subject: MGG and blue artifact

Hello fellow histonetters,
      Would anyone have any thoughts on why mggs sometimes will result in a very blue slide (not good)?  My understanding is possible formaldehyde exposure, or smear inadequately dried before being fixed.  What other reasons might there be?   We pH our buffer.  Any thoughts and help are appreciated.  Thanks.

Deb King, HT (ASCP)
Sacramento, CA

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