RE: Keratin

I don't agree. Acids swell tissue more than water, and it's the added
swelling that 'softens'.  I have sectioned many a uterine fibroid and cone
biopsy of cervix in my time that required formic acid in addition to the
iced water routinely used to get a good section.

                    "Marshall Terry Dr,                                                                                                               
                    Consultant                   To:     Stephen Eyres/GB-ALNWICK/RESEARCH/SANOFI@Research                                            
                    Histopathologist"            "Histonet"                                                             
                                       Subject:     RE: Keratin                                                                             
                    25/07/2002 13:45                                                                                                                  

If 10% formic acid does anything it's because 10% formic acid is 90% water.

Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire

-----Original Message-----
Sent: 25 July 2002 08:42
To: Histonet
Subject: Re: Keratin

I agree with Aidan. 10% formic acid also works well, or whatever
decalcifying agent you use.

                    Aidan Schurr
                    25/07/2002            Subject:     Re: Keratin

Can't beat Mollifex in my opinion.



aidan schurr
section head, histology
hutt valley district health board
lower hutt
new zealand
++64 4 570 9173 (direct)
++64 4 570 9214 (fax)

>>> Valerie Hannen  25/07/2002 >>>
Hi fellow histonetters!!!!

Again, I have a question. Is there a "miracle" solution one can use to
soften finger and toenails for sectioning purposes? We received some really
nasty/hard ones. We tried a dilute ammonium hydroxide solution it helped a
but we hoping to find something better.

Any help you can give me would be greatly appreciated.


Valerie Hannen
Parrish Medical Center
Titusville,Florida 32927

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