RE: Keratin

From:Stephen.Eyres@sanofi-synthelabo.com


I don't agree. Acids swell tissue more than water, and it's the added
swelling that 'softens'.  I have sectioned many a uterine fibroid and cone
biopsy of cervix in my time that required formic acid in addition to the
iced water routinely used to get a good section.
Steve


                                                                                                                                                      
                    "Marshall Terry Dr,                                                                                                               
                    Consultant                   To:     Stephen Eyres/GB-ALNWICK/RESEARCH/SANOFI@Research                                            
                    Histopathologist"            "Histonet"                                                             
                                       Subject:     RE: Keratin                                                                             
                                                                                                                                                      
                    25/07/2002 13:45                                                                                                                  
                                                                                                                                                      
                                                                                                                                                      




If 10% formic acid does anything it's because 10% formic acid is 90% water.

Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire
terry.marshall@rgh-tr.trent.nhs.uk

-----Original Message-----
From: Stephen.Eyres@sanofi-synthelabo.com
[mailto:Stephen.Eyres@sanofi-synthelabo.com]
Sent: 25 July 2002 08:42
To: Histonet
Subject: Re: Keratin



I agree with Aidan. 10% formic acid also works well, or whatever
decalcifying agent you use.
Steve



                    Aidan Schurr
                                histonet@pathology.swmed.edu
                                          cc:
                    25/07/2002            Subject:     Re: Keratin
                    04:17






Can't beat Mollifex in my opinion.

Aidan


__

aidan schurr  b.m.l.sc
section head, histology
hutt valley district health board
lower hutt
new zealand

aidan.schurr@hvh.co.nz
++64 4 570 9173 (direct)
++64 4 570 9214 (fax)

>>> Valerie Hannen  25/07/2002 >>>
Hi fellow histonetters!!!!

Again, I have a question. Is there a "miracle" solution one can use to
soften finger and toenails for sectioning purposes? We received some really
nasty/hard ones. We tried a dilute ammonium hydroxide solution it helped a
little,
but we hoping to find something better.


Any help you can give me would be greatly appreciated.


Thanks

Valerie Hannen
Parrish Medical Center
Titusville,Florida 32927













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