RE: Breast Core bx. IHC

From:"Horn, Hazel V"

Just a thought but it could be drying out a bit before they get it into
fixative.   This would cause non specific staining to happen.
So, it just may not be anything you or your lab is doing.   
Hazel

> -----Original Message-----
> From:	Dawson, Glen [SMTP:GDawson@Milw.Dynacare.com]
> Sent:	Tuesday, July 23, 2002 11:18 AM
> To:	HistoNet Server
> Subject:	RE: Breast Core bx. IHC
> 
> All,
>  
> I was curious as to whether or not anyone else is experiencing a marked
> difference in nonspecific IHC staining between breast core biopsies and
> large pieces of breast.  I am running into alot of nonspecific cytoplasmic
> staining on my breast core biopsies that does not occur on the larger
> pieces
> of breast from the same case.  We have attempted different processing
> times
> to prevent it but to no avail.   The biggest difference between the
> handling
> of the breast Bigs and the breast Bx's is the processing times.  My logic
> is
> that the core biopsy should not need the extended processing due to it's
> small size.  I'm at a loss.  Any like experiences or suggestions would be
> appreciated.
>  
> Thanx in Advance,
>  
> Glen Dawson  BS, HT &  IHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
> 




<< Previous Message | Next Message >>