RE: Breast Core bx. IHC
Just a thought but it could be drying out a bit before they get it into
fixative. This would cause non specific staining to happen.
So, it just may not be anything you or your lab is doing.
Hazel
> -----Original Message-----
> From: Dawson, Glen [SMTP:GDawson@Milw.Dynacare.com]
> Sent: Tuesday, July 23, 2002 11:18 AM
> To: HistoNet Server
> Subject: RE: Breast Core bx. IHC
>
> All,
>
> I was curious as to whether or not anyone else is experiencing a marked
> difference in nonspecific IHC staining between breast core biopsies and
> large pieces of breast. I am running into alot of nonspecific cytoplasmic
> staining on my breast core biopsies that does not occur on the larger
> pieces
> of breast from the same case. We have attempted different processing
> times
> to prevent it but to no avail. The biggest difference between the
> handling
> of the breast Bigs and the breast Bx's is the processing times. My logic
> is
> that the core biopsy should not need the extended processing due to it's
> small size. I'm at a loss. Any like experiences or suggestions would be
> appreciated.
>
> Thanx in Advance,
>
> Glen Dawson BS, HT & IHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
>
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