All,
 
I was curious as to whether or not anyone else is experiencing a marked
difference in nonspecific IHC staining between breast core biopsies and
large pieces of breast.  I am running into alot of nonspecific cytoplasmic
staining on my breast core biopsies that does not occur on the larger pieces
of breast from the same case.  We have attempted different processing times
to prevent it but to no avail.   The biggest difference between the handling
of the breast Bigs and the breast Bx's is the processing times.  My logic is
that the core biopsy should not need the extended processing due to it's
small size.  I'm at a loss.  Any like experiences or suggestions would be
appreciated.
 
Thanx in Advance,
 
Glen Dawson  BS, HT &  IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI