Mouse testes processing

From:"Johnson, Teri"

Hello all!

I'm having difficulty with routine processing of mouse testes.  I haven't been able to preserve the intertubular connective tissue.  I've tried bouins fixative and it's well preserved.  It mostly disappears with 4% paraformaldehyde and 10% neutral buffered formalin.  The simple answer is to use bouins, but what I'd really like to do is figure out how to preserve it with routine fixation/processing.

My processor is set up as follows:
70% alcohol
Prosoft x5 stations
ProPar x3 stations
paraffin x4 stations

I will post a picture to the site and will send another email with the path when it's posted.

Also, for those of you using Davidson's fixative on mouse tissues, have there been many problems with subsequent immunostaining?

Thanks for your help,

Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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