Iron Stains
I seem to have a problem seeing Iron in my Bone
Marrow clot sections. My control always works- it is a purchased positive
control that is formalim fixed. I use B-5 to fix my clots and the bone bx. The
direct asperate smear shows iron most of the time and that is fixed with methyl
alcohol. this smear is air dried after the iron stain water rinse. My clot
sections are brought through alcohols and xylene and then coverslipped. Am i
washing out my iron in the Alcohols? Is there anyone who uses B-5 for bone
marrow fixative? What is the secret? please help me.
Joyce Walter
Warren General Hospital
Warren, Pa
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