IHC in frozen mouse liver
I am trying to establish a protocol for immunohistochemistry in
frozen mouse liver sections in our lab. I would first like to know
what antibody to use as a good positive control (for liver) to
determine whether a procedure is working in current and/or future
experiments. I would also like to ask anyone who has successfully
performed such an experiment to email any protocols, sources of
information or knowledge they may have on the subject. I'll name my
first born after you or give you a kidney.
Additionally, I have tried the Vector ABC detection kit only to
recieve a high background (even after A/B blocking) and I have so far
not had any trouble with endogenous peroxidases showing background(is
this normal?). I have recently tried IHC using a SRB1 primary antibody
and detection with an HRP labelled secondary with the results as
follows:
SRB1 (IgG from rabbit)= good signal
Neg.control(No primary)= no signal, very clean
Neg.control(serum IgG from a non-innoculated rabbit)= low signal
Seeing as my non-innoculate negative control was not clean I am unsure
about the results. Suggestions? Comments? Thank you very much for
any help and time you provide,
JohnM.
Research Assistant II
UTatHSC
<< Previous Message | Next Message >>