GFP and bone sections
Hi there,
we are facing a problem preserving GFP (EGFP, DsRed, Clontech) visualization
in bone sections. To our knowledge we have to perform a decalcification. Does
anybody has experience with a protocol leaving GFP visualization intact ?
Should we use EDTA, formic acid ? In which concentrations ? Preferably we
would like to perform cryosections rather than parraffin processing. Does
anybody has experience with a method preparing fresh frozen sections of
undecalcified tissues (Kawamoto T. and Masaharu S. Histochem Cell Biol 2000,
113: 331-339).
Any help on this problem would be very appreciated.
Martin Weber
The Scripps Resaerch Institute
10550 North Torrey Pines Road
La Jolla, 92037 C.A.
Phone: 858-784-8612
E-Mail: maweber@scripps.edu
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