GFP and bone sections
we are facing a problem preserving GFP (EGFP, DsRed, Clontech) visualization
in bone sections. To our knowledge we have to perform a decalcification. Does
anybody has experience with a protocol leaving GFP visualization intact ?
Should we use EDTA, formic acid ? In which concentrations ? Preferably we
would like to perform cryosections rather than parraffin processing. Does
anybody has experience with a method preparing fresh frozen sections of
undecalcified tissues (Kawamoto T. and Masaharu S. Histochem Cell Biol 2000,
Any help on this problem would be very appreciated.
The Scripps Resaerch Institute
10550 North Torrey Pines Road
La Jolla, 92037 C.A.
<< Previous Message | Next Message >>