GFP and bone sections


Hi there,

we are facing a problem preserving GFP (EGFP, DsRed, Clontech) visualization 
in bone sections. To our knowledge we have to perform a decalcification. Does 
anybody has experience with a protocol leaving GFP visualization intact ? 
Should we use EDTA, formic acid ? In which concentrations ? Preferably we 
would like to perform cryosections rather than parraffin processing. Does 
anybody has experience with a method preparing fresh frozen sections of 
undecalcified tissues (Kawamoto T. and Masaharu S. Histochem Cell Biol 2000, 
113: 331-339).

Any help on this problem would be very appreciated.

Martin Weber
The Scripps Resaerch Institute
10550 North Torrey Pines Road
La Jolla, 92037 C.A.
Phone: 858-784-8612

<< Previous Message | Next Message >>