GFP again (sigh)

From:"Johnson, Teri"

I've searched the archives previously and have just done so
again.  We're trying to visualize GFP in mouse tissues.
The 4% paraformaldehyde fixed, formic acid decalcified,
paraffin embedded bone specimens show "staining" in every cell.  
The unfixed, frozen skin specimens show "staining" only in the
hair shafts.

We are using EGFP. We are not using nail polish to seal the
coverslips, nor are we using aqueous mounting medium (fading
from the PVA).

It's apparent many others have had these same problems and I'm 
interested to know how they were solved.  Donna Montague has had
great fortune in obtaining good results with eGFP.  I'm wondering
if maybe walking next to her or something might help some
of this rub off on me?

Thanks for your help!

Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

<< Previous Message | Next Message >>